Inositol-Stabilized Arginine Silicate Improves Post-Exercise Cognitive Function in Recreationally Active, Healthy Males: A Randomized, Double-Blind, Placebo-Controlled Crossover Study

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Malkanthi Evans
Alison C McDonald
David C Crowley
Nisrine Zakaria
Najla Guthrie


Cognitive flexibility, Sports performance, Nitric oxide


Introduction: At all competition levels, athletic performance depends on simultaneously handling physiological and cognitive demands. The objective of this study was to determine the efficacy of a patented complex of inositol-stabilized arginine silicate (ASI; Nitrosigine®) to improve cognitive performance following intense exercise.
Methods: Twenty-four healthy, recreationally active males (26.50 ± 4.85 years, 25.31 ± 2.52 kg/m2 BMI) were enrolled in this randomized, double-blind, placebo-controlled crossover study. A single oral dose of 1500 mg ASI or placebo was administered 30 minutes before a maximally graded exercise test. Prior to and following exercise, cognitive function was assessed via the Trail Making Test (TMT). Safety was evaluated at end of study.
Results: Following intense exercise, ASI reduced the time to complete TMT Part A and B (2.80 ± 5.97, 5.54 ± 3.77 seconds, respectively) compared to increased time with placebo (19.82 ± 4.58, 7.68 ± 5.06 seconds, respectively). Differences between ASI and placebo were statistically significant (p = 0.01 TMT A, p = 0.05 TMT B). ASI was found to be safe and well- tolerated with no related adverse events.
Conclusion: Intense exercise resulted in impaired cognitive function, which was prevented by pre-exercise ASI consumption. Future research should examine the efficacy of ASI in athletes, as improvement in cognitive function would be advantageous through enhanced ability to process complex information in quickly changing games or events.

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